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假复层纤毛柱状上皮

2016-06-14 09:26阅读:
假复层纤毛柱状上皮(14号,气管,H-E染色)
1.肉眼观察:在气管内表面被染成紫蓝色的一层即假复层纤毛柱状上皮。
2.低倍观察:上皮组织核染成紫蓝色,高低不等。细胞分界不明显。
3.高倍观察:可见假复层纤毛柱状上皮由高矮不等的四种细胞组成,其形态特点如下:
柱状细胞:数量最多,形似柱状,顶部可达游离面,在其表面可见有一排纤细而整齐的纤毛,胞核椭圆形,位于细胞顶部,故排列在整个上皮的表层。
杯状细胞:夹在柱状细胞之间,顶部胞质呈白色空泡状,有时可呈浅蓝色。
梭状细胞:位于柱状细胞与锥体状细胞之间,胞体呈梭状,胞核椭圆形,位于细胞中央,故排列在整个上皮的中层。
锥体状细胞:细胞较小,呈锥体形,其基底面紧贴在基膜上,胞核圆形,位于细胞中央,在整个上皮中为紧贴基底面的一层胞核。
基膜:紧贴上皮基底面,为一层均质状粉红色的薄膜。
Pseudostratified ciliated columnar epithelium (No 14, trachea, H&E)
1. Naked eye: The concave surface is purple and lined w
ith epithelium.
2. Low power: Many nuclei are close to each other and at different levels.
3. High power: There are approximately 3 layers of nuclei giving a stratified appearance. The upper nuclei are of columnar cells whose free surface possesses a number of cilia. The nuclei in the middle are of fusiform cells. The nuclei at the bottom are of pyramidal cells. Goblet cells are also present. Note that there is an obvious basement membrane just beneath the epithelium. All epithelial cells rest on the basement membrane and the stratified appearance results from the fact that the cells vary in height and not all of them reach the free surface.

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