- 把T75细胞培养瓶从常氧环境转移到2%的低氧环境后，需要约24小时培养基中的氧浓度才达到2%。
- 如在常氧环境中进行换液操作，仅4min后，培养基中氧浓度就从0%升至8.5%，而使其恢复到2%低氧则需约16h。
《Dissolved Oxygen Concentration in Culture Medium: Assumptions and Pitfalls》
新浪博客
《Dissolved Oxygen Concentration in Culture Medium: Assumptions and Pitfalls》
确控制或为了检测培养基溶氧的客户，Biospherix提供OxyValidator溶氧检测仪，可以用于检测多种培养器皿中的溶氧。
工作原理：
Fiber Optic Dissolved Oxygen Sensor
This is accomplished by utilizing a special fiber optic oxygen probe that has an inert fluorescent thin-film coating on the probe tip.
The probe tip is mounted on a stage above the culture vessel such that it can be manually inserted into the media in any standard culture dish or flask with either a small hole drilled in the cover or topside surface (or dish without a cover), and retracted manually as necessary. The probe tip must be removed from the culture chamber manually when the chamber is removed from host incubator to be transported to a glove box. The entire dissolved oxygen sensing system will remain in the host incubator while the culture chamber is removed, and the probe tip must be manually inserted back into the stage when the culture chamber is returned to the host incubator.
工作原理：
Fiber Optic Dissolved Oxygen Sensor
This is accomplished by utilizing a special fiber optic oxygen probe that has an inert fluorescent thin-film coating on the probe tip.
The probe tip is mounted on a stage above the culture vessel such that it can be manually inserted into the media in any standard culture dish or flask with either a small hole drilled in the cover or topside surface (or dish without a cover), and retracted manually as necessary. The probe tip must be removed from the culture chamber manually when the chamber is removed from host incubator to be transported to a glove box. The entire dissolved oxygen sensing system will remain in the host incubator while the culture chamber is removed, and the probe tip must be manually inserted back into the stage when the culture chamber is returned to the host incubator.