【揭秘】细胞转染的正确打开方式,你选对了吗?
2022-05-05 10:07阅读:
还在为转染实验发愁吗?
细胞太脆弱,经不住折腾,
不能含血清,苦命地反复换液,
转染效率低,实验各种不理想。
面对一次次的挫败,
什么时候我才能翻身做主人,
漂漂亮亮转细胞。
细胞转染试剂
STRONG>
全式金转染试剂EL、PL,凭借自身高效的产品性能,超强的细胞广适性,助你轻松应对转染实验!
TransIntro® EL Transfection
Reagent
该产品是一种非脂质体转染试剂,适用于将DNA和RNA转入真核细胞。该试剂转染效率高,细胞毒性低,转染时可以有血清和抗生素的存在,对贴壁细胞和悬浮细胞均可转染。
1、产品特点
转染效率高:提升对多种难转染细胞的转染效率。
细胞毒性低:对细胞生理过程影响小。
操作简单:转染后无需更换培养基,可带血清和抗生素转染。
适用范围广:DNA和RNA均可转染。
2、实验数据

3、已成功转染细胞

TransIntro® PL Transfection
Reagent
该产品是一种脂质体转染试剂,适用于将DNA转入真核细胞,针对应用广泛的难转染细胞,有效提高DNA的转染效率。
1、产品特点
对于肺癌、结直肠癌、肝癌、胰腺癌等多种肿瘤细胞系的转染高效稳定,有助于建立稳定可靠的细胞模型。
细胞毒性低,对细胞生理过程影响小。
抗生素对转染效率没有影响。
2、实验数据

3、已成功转染细胞

产品信息

参与研究领域
全式金转染试剂自上市以来,备受广大科研工作者的青睐,目前已被广泛应用于多个研究领域。
1、
胆固醇衍生物诱导细胞自噬的机制研究

2、先天免疫反应研究
本研究揭示了RUVBL1/2作为巨噬细胞促炎反应的重要组成部分,参与表观遗传调控的分子机制,以及在促炎通路被异常激活引发疾病的过程中,RUVBL1/2抑制剂表现出一定的治疗潜能,为先天免疫的研究提供了新思路,其中转染细胞为RAW
264.7巨噬细胞。

3、癌细胞调控机制研究
本研究揭示了CIB-6通过靶向STAT3的SH2结构域,可抑制STAT3的磷酸化和核易位,进而调控STAT3介导的β-TrCP/β-catenin/NF-κB轴过程,在体内外有效抑制HCC肿瘤的发生,为HCC的治疗提供新策略,其中转染细胞为HepG2细胞。

4、抗心肌肥大的靶点研究
本研究中,通过调控miR-214的表达,抑制SIRT3参与血管紧张素II诱导的心肌肥大,进而导致线粒体功能障碍,揭示了miR-214可能为抗心肌肥大治疗的一个潜在的干预靶点,其中转染细胞为心肌细胞AC16和NRCMs。

5、抗病毒治疗的新细胞靶点研究
本研究揭示了mTOR信号通路通过PGC-1α依赖的SIRT3调控ISKNV感染细胞的还原性谷氨酰胺代谢,为ISKNV的发病机制和抗病毒治疗策略提供了新的见解,其中转染细胞为CPB细胞。

助力发表的部分文章
• Fu X, Li K, Niu Y, et al. The
mTOR/PGC-1α/SIRT3 Pathway Drives Reductive Glutamine Metabolism to
Reduce Oxidative Stress Caused by ISKNV in CPB Cells[J].
Microbiology Spectrum, 2022, 10(1): e02310-21.
• Wu W, Luo M, Li K, et al.
Cholesterol derivatives induce dephosphorylation of the histone
deacetylases Rpd3/HDAC1 to upregulate autophagy[J]. Autophagy,
2021, 17(2): 512-528.
• Zhang R, Cheung C Y,
Seo S U, et al. RUVBL1/2 Complex Regulates Pro-Inflammatory
Responses in Macrophages via Regulating Histone H3K4
Trimethylation[J]. Frontiers in immunology, 2021, 12:
2074.
• Liu Y, Cheng A, Wang M, et al. Duck
Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent
Cellular Translation Shutoff via PERK/GCN2[J]. Frontiers in
microbiology, 2021, 12: 746.
• Ding Y, Zhang Y, Lu J, et al.
MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by
targeting SIRT3 to provoke mitochondrial malfunction[J]. Acta
Pharmacologica Sinica, 2021, 42(9): 1422-1436.
• Jiang J,
Liu T, He X, et al. Silencing of KIF18B restricts proliferation and
invasion and enhances the chemosensitivity of breast cancer via
modulating Akt/GSK3β/βcatenin pathway[J]. BioFactors, 2021, 47(5):
754-767.
• Peng T, Wonganan O,
Zhang Z, et al. A 2-Benzylmalonate Derivative as STAT3 Inhibitor
Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating
β-TrCP E3 Ubiquitin Ligase[J]. International journal of molecular
sciences, 2021, 22(7): 3354.
• Chen Y, Zhu S, Hu J,
et al. gga-miR-1603 and gga-miR-1794 directly target viral L gene
and function as a broad-spectrum antiviral factor against NDV
replication[J]. Virulence, 2021, 12(1): 45-56.
• Hu J, Wang J, Li C, et
al. Fructose1, 6bisphosphatase aggravates oxidative stressinduced
apoptosis in asthma by suppressing the Nrf2 pathway[J]. Journal of
cellular and molecular medicine, 2021, 25(11):
5001-5014.
• Cui Q, Yan L.
Tripartite motif-containing protein 16 protects against myocardial
ischemia/reperfusion injury by affecting the Keap1/Nrf2 axis[J].
Cell and tissue research, 2021, 386(2): 349-363.
• Wu W, Li K, Guo
S, et al. P300/HDAC1 regulates the acetylation/deacetylation and
autophagic activities of LC3/Atg8–PE ubiquitin-like system[J]. Cell
death discovery, 2021, 7(1): 1-15.
• Hu S, Li Y, Wang
B, et al. TRIM38 protects chondrocytes from IL-1β-induced apoptosis
and degeneration via negatively modulating nuclear factor (NF)-κB
signaling[J]. International Immunopharmacology, 2021, 99:
108048.
• Zhu F, Shao J, Tian Y,
et al. Sulfiredoxin-1 protects retinal ganglion cells from high
glucose-induced oxidative stress and inflammatory injury by
potentiating Nrf2 signaling via the Akt/GSK-3β pathway[J].
International Immunopharmacology, 2021, 101: 108221.
• Zhang L, Zhu T, He F,
et al. Senescence marker protein 30 (SMP30) protects against high
glucose-induced apoptosis, oxidative stress and inflammatory
response in retinal ganglion cells by enhancing Nrf2 activation via
regulation of Akt/GSK-3β pathway[J]. International
Immunopharmacology, 2021, 101: 108238.
• Xiang C, Huang M,
Xiong T, et al. Transcriptomic analysis and functional
characterization reveal the duck interferon regulatory factor 1 as
an important restriction factor in the replication of Tembusu
virus[J]. Frontiers in microbiology, 2020: 2069.
• Sun Y, He L, Wang T,
et al. Activation of p62-Keap1-Nrf2 pathway protects
6-hydroxydopamine-induced ferroptosis in dopaminergic cells[J].
Molecular Neurobiology, 2020, 57(11): 4628-4641.
• Wang T, Merits A, Wu
Y, et al. Cis-acting sequences and secondary structures in
untranslated regions of duck tembusu virus RNA are important for
cap-independent translation and viral proliferation[J]. Journal of
Virology, 2020, 94(16): e00906-20.
• Yuan L X, Liang J Q,
Zhu Q C, et al. A gammacoronavirus, avian infectious bronchitis
virus, and an alphacoronavirus, porcine epidemic diarrhea virus,
exploit a cell survival strategy by upregulating cFOS to promote
virus replication[J]. Journal of Virology, 2020, 95(4):
e02107-20.
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