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ATCC9610小肠结肠炎耶尔森菌亚种的实验室检查！

2024-09-10 16:45阅读：

http://blog.sina.cn/dpool/blog/u/7058810482

ATCC 9610小肠结肠炎耶尔森菌亚种的实验室检查！

小肠结肠炎耶尔森氏菌属肠杆菌科，最适生长温度为22～29，在0也能生长。有34种O抗原，19种H抗原和1种K抗原，由抗原组成可分成许多菌群。从病人分离出的菌型大多数为O3型，少数为O5、O8、O9和混合型。

一、菌种简介
平台编号：Bio-75128
规格：Freeze-Dried
拉丁属名：Yersinia Enterocolitica Subsp. Enterocolitica
菌株名称：小肠结肠炎耶尔森菌亚种
用途：ATCC原装进口
注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）


二、详细描述
Yersinia enterocolitica subsp.菌株拉丁名
enterocolitica (Schleifstein and Coleman) Frederiksen
(ATCC® 9610™)菌株编号
Strain Designations菌株别名: 33114 [CCUG 11291, CCUG 12369, CIP 80.27, DSM 4780, LMG 7899, NCTC 12982] /
Type Strain模式菌株: yes /
Biosafety Level生物安全等级: 2
Deposited As Bacterium enterocoliticum Schleifstein and Coleman Classification Enterobacteriaceae, Yersinia
Strain Designations菌株别名 33114 [CCUG 11291, CCUG 12369, CIP 80.27, DSM 4780, LMG 7899, NCTC 12982]
Application Media testing Pharmaceutical and Personal Care
Isolation 分离源Tissue, human; glanders-like infection of the face New York, United States
Biosafety Level生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties biovar 1, serogroup O:8 Ref
Product Format提供形式 freeze-dried
Storage Conditions Frozen保藏方法: -80 or colder
Freeze-Dried冻干物: 2 to 8
Live Culture活菌: See Propagation Section
Preceptrol® no
Type Strain模式菌株 yes Ref, Ref, (Frederiksen WA study of some Yersinia pseudotuberculosis-like bacteria (Bacterium enterocoliticum and Pasteurella X).In: Frederiksen WProceedings of the XIV Scandinavian Congress of Pathology and Microbiology, Oslo 1964OsloNorwegian Universities Press103-104, 1964) Antigenic Properties biovar 1, serogroup O:8 Ref
Comments注释 Phagovar Xz Sal/Esc-Pyz-CRMOX+
Medium培养基 ATCC® Medium 44: Brain Heart Infusion Agar/Broth
Growth Conditions生长条件
Temperature培养温度: 30
Atmosphere需氧情况: Aerobic
Name of Depositor JM Coffey, RM Pike
Isolation分离源 Tissue, human; glanders-like infection of the face New York, United States
References参考文献 Bergey's Manual Syst. Bacteriol. 1: 505, 1984.
Schleifstein JI, Coleman MB. An unidentified microorganism resembling B. ligniere and Past. pseudotuberculosis, and pathogenic for man. N.Y. State J. Med. 39: 1749-1753, 1939.
Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Validation list no. 75. Int. J. Syst. Evol. Microbiol. 50: 1415-1417, 2000. McIver MA, Pike RMChronic glanders-like infection of face caused by an organism resembling Flavobacterium pseudomallei WhitmoreIn: McIver MA, Pike RMClinical miscellany1Springfield, ILCharles C.
Thomas16-21, 1934 Frederiksen WA study of some Yersinia pseudotuberculosis-like bacteria (Bacterium enterocoliticum and Pasteurella X).In: Frederiksen WProceedings of the XIV Scandinavian Congress of Pathology and Microbiology, Oslo 1964OsloNorwegian Universities Press103-104, 1964 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

三、细菌的实验室检查及其它检查
1、标本采集常为粪便以及食物，也可取血液和尿液等。
粪便标本采集要求：采集的动物粪便标本应新鲜，病人的粪便标本最好在发病3-5病日内，使用抗生素之前。粪便采集量每份不少于5克，置于干净塑料袋或置于运送培养基中于6小时内送实验室检测，夏季标本的保存和运送要注意冷藏。原则上每天采集的标本可分两次送至实验室，即上午的标本中午12时前送到，下午的标本晚6时前送到，以便于及时开展检测医`学教育网搜集整理。
2、分离培养——以粪便标本为例
（1）冷增菌粪便与改良磷酸盐缓冲液以1：10混合于4增菌，并于10天，20天分别转种麦康凯MAC平板或耶尔森菌属专用选择性培养基CIN于25培养24～48h.
（2）该菌的菌落在MAC平板上呈无色透明，在CIN平板上呈粉红色，偶尔有一圈胆盐沉淀；
（3）将可疑菌落接种改良克氏双糖培养基25培养24h～48h，上、下层均为阳性（即显黄色）者接种Rustigian氏尿素培养基（使成浓厚菌悬液），振荡10秒钟25培养2～4h.
（4）尿素阳性者再接种半固体各2管，1管于25培养24h，另1管于37培养24h.该菌在25动力阴性，37动力阴性。
3、生化鉴定（25培养）：典型小肠结肠炎耶尔森菌主要的生化反应情况：脲酶阳性、硫化氢阴性、VP25阳性、鸟氨酸脱羧酶阳性。发酵葡萄糖、蔗糖产酸不产气，不发酵密二糖和鼠李糖。
4、血清分型。

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